AINI

Menu
AINI CONGRESS
AINI CONGRESS
AINI CONGRESS
AINI CONGRESS
Menu

Research Units

Avolio Carlo MD

Coordinator Neuroimmunology Unit
Laboratory of Experimental and Regenerative Medicine

Department of Medical and Surgical Sciences

University of Foggia

Viale Luigi Pinto, 1
71100 Foggia – Italy
Tel. 0881 736080 – 0881 588 097

Collaboratori

Keywords su ambiti di competenza:
Multiple Sclerosis; disease modifying drugs; extracellular vesicles; monocytes; dendritic cells; T-cell subsets; purinergic receptors; cerebrospinal fluid; blood-brain barrier; adhesion molecules; cytokines; MMPs
“Cellular Biology: Flow cytofluorimetry for several cellular analysis; epifluorescence microscopy;
human peripheral blood cell purification (monocytes, lymphocytes); primary cell and cell line culture;
cell counting; extracellular vesicles and subcellular organelles isolation and purification.

 

Tecniche utilizzate:
Biochemistry
Potentiometry and pH measurements; protein extraction and purification; Bradford and Lowry protein assay; enzymatic activity assay; Western blot; gel electrophoresis; SDS-PAGE;
Immunoprecipitation; ELISA; fluorimetric, spectrophotometric and oxygraphic measurements;
Immunoassays; Immunohistochemistry; Immunocytochemistry.

 

Molecular Biology
Nucleic acids purification and quantification (DNA, RNA) from cells and tissues;
DNA and RNA extraction, purification and PCR-amplification; qRT-PCR; Real Time PCR; gene silencing.

Ballerini Clara PhD

Associate Professor, coordinator

Laboratory of Neuroimmunology
Department of Experimental and Clinical Medicine

University of Florence

Viale Pieraccini, 6
50139 Firenze – Italy
Tel. 055 2758252

Collaboratori
  • Roberta Amoriello, post doc
  • Alberto Carnasciali, PhD student
  • Nina Costanza Boscolo, student
  • Zainab Soltani, student
  • Giulia Storti, student

Keywords su ambiti di competenza:
DCs, MSCs, T lymphocytes, TCR repertoire, CKs analysis, EAE, MS, Nanotechnology.

 

Tecniche utilizzate:

High throughput sequencing and analysis of TCR and BCR, Cell Biology Assays, Molecolar Biology Assays, Confocal Microscopy, Electron Microscopy, Flow Citometry, Luminex Technology, Immunohistochemistry, Histology, Mice performance tests

Battistini Luca

Head, Neuroimmunology Unit

Unità di Neuroimmunologia
Dipartimento di Neuroscenze Sperimentali

I.R.C.C.S. Fondazione Santa Lucia

Via del Fosso di Fiorano, 64
00143 Roma – Italia
Tel. 06 50170 3090
Fax 06 50170 3326

Collaboratori
  • Giovanna Borsellino, Responsabile dell’Unità di Citofluorimetria
  • Roberta Placido, Dirigente Biologo
  • Daniela Angelini, Dirigente Biologo
  • Elisabetta Volpe, Dirigente Biologo
  • Manolo Sambucci, Dottore di Ricerca
  • Gabriella Ruocco, Dottore di Ricerca
  • Marco De Bardi, Dottorando
  • Gisella Guerrero, Dottoranda
  • Francesca Gargano, Dottoranda
  • Manuela Bianco, Borsista

Keywords su ambiti di competenza:
Autoimmunity, T cells, Innate immunity, Multiple Sclerosis, Flow Cytometry.

 

Tecniche utilizzate:

Citofluorimetria, ELISA, Western Blot, Colture cellulari, live imaging.

Bonetti Bruno

Head, Neuroimmunology Unit

Laboratorio di Neuropatologia

Dipartimento di Scienze Neurologiche e del Movimento – Università di Verona

Ospedale Policlinico GB Rossi
Piazza L. Scuro, 10
37134 Verona – Italia
Tel. 045 812 4694

Collaboratori
  • Alessia Farinazzo, Assegnista
  • Ermanna Turano, Assegnista
  • Elena Bazzoli, Assegnista

Keywords su ambiti di competenza:
Adipose Stem Cells, Proteomics, Nanovesicles.

 

Tecniche utilizzate:

Colture cellulari, proteomica, immunoistochimica, microscopia ottica, microscopia confocale, microscopia elettronica.

Coccia Eliana Marin

Head, Anti-infectious Immunity Unit

Unità di Immunità anti-infettiva

Istituto Superiore di Sanità

Viale Regina Elena, 299
00161 Roma – Italia
Tel/Fax 06 499 036 38

Collaboratori
  • Elena Giacomini, Tecnica laureata
  • Martina Severa, Ricercatore a tempo determinato
  • Marilena Paola Etna, Borsista
  • Fabiana Rizzo, Borsista
  • Melania Cruciani, PhD student

Keywords su ambiti di competenza:
Monocyte-derived and plasmacytoid Dendritic cells, B cells, TLR signaling, Type I IFN regulation.

 

Tecniche utilizzate:

Cell culture, multi-parametric flow cytometry, gene expression analysis by quantitative real time PCR, protein analysis by electrophoresis, ELISA and cytokine bead array

Cosentino Marco

Head, Neuroimmune Pharmacology Unit

Unità di Neuroimmuno Farmacologia

Centro Interdipartimentale di Ricerche in Farmacologia Medica
Università degli Studi dell’Insubria Varese-Como

Via Ottorino Rossi, 9
21100 Varese – Italia
Tel. 0332 217401
Fax. 0332 217409

Collaboratori
  • Franca Marino, Ricercatore
  • Marco Ferrari, Ricercatore
  • Emanuela Rasini, Tecnico (citofluorimetria)
  • Raffaella Bombelli, Tecnico (HPLCe metodiche spettrofluorimetriche)
  • Alessandra Luini, Tecnico (colture cellulari)
  • Massimiliano Legnaro, Tecnico (biologia molecolare)
  • Natasa Kustrimovic, post-doc
  • Angela Scanzano, post-doc

Keywords su ambiti di competenza:
dopamine, noradrenaline, adrenaline, T lymphocytes, neutrophils, multiple sclerosis, Parkinson’s disease, rheumatoid arthritis.

 

Tecniche utilizzate:

Citometria a flusso, colture cellulari, real time PCR, spettrofluorimetria, HPLC-ED, HPLC-UV, ELISA.

Pubblicazioni 2010-2015

  1. Angela Scanzano, Laura Schembri, Emanuela Rasini, Alessandra Luini, Jessica Dallatorre, Massimiliano Legnaro, Raffaella Bombelli, Terenzio Congiu, Marco Cosentino, Franca Marino. Adrenergic modulation of migration, CD11b and CD18 expression, ROS and interleukin-8 production by human polymorphonuclear leukocytes. Inflammation Research 2015, 64: 127-135.

  2. Natasa Kustrimovic, Emanuela Rasini, Massimiliano Legnaro, Franca Marino, Marco Cosentino. Expression of dopaminergic receptors on human CD4+ T lymphocytes: flow cytometric analysis of naive and memory subsets and relevance for the neuroimmunology of neurodegenerative disease. Journal of Neuroimmune Pharmacology 2014, 9: 302-312.

  3. Silvia Capellino, Marco Cosentino, Alessandra Luini, Raffaella Bombelli, Torsten Lowin, Maurizio Cutolo, Franca Marino, Rainer H. Straub. Increased expression of dopamine receptors in synovial fibroblasts of patients with rheumatoid arthritis – Inhibitory effects of dopamine on IL-8 and IL-6. Arthritis & Rheumatology 2014, 66: 2685-2693.

  4. Marco Cosentino, Mauro Zaffaroni, Franca Marino. Levels of mRNA for dopaminergic receptor D5 in circulating lymphocytes may be associated with subsequent response to interferon-β in patients with multiple sclerosis. Journal of Neuroimmunology 2014, 277: 193-196.

  5. Marco Cosentino, Franca Marino. Adrenergic and dopaminergic modulation of immunity in multiple sclerosis: teaching old drugs new tricks? Journal of Neuroimmune Pharmacology 2013, 8: 163-179.

  6. Franca Marino, Marco Cosentino. Adrenergic modulation of immune cells: an update. Amino Acids 2013, 45: 55-71.

  7. Marco Cosentino, Howard E. Gendelman. The First Insubria Autumn School on Neuroimmunopharmacology: challenging paradigms beyond boundaries. Journal of Neuroimmune Pharmacology 2013, 8: 1-3.

  8. Marco Cosentino, Franca Marino, Salvatore Cuzzocrea, Monica Di Luca. Neuroimmune pharmacology: an emerging discipline for the Italian Society of Pharmacology. Journal of Neuroimmune Pharmacology 2013, 8(Suppl 1): S1-S3.

  9. Marco Cosentino, Mauro Zaffaroni, Maria Trojano, Maurizio Giorelli, Carmela Pica, Emanuela Rasini, Raffaella Bombelli, Marco Ferrari, Angelo Ghezzi, Giancarlo Comi, Paolo Livrea, Sergio Lecchini, Franca Marino. Dopaminergic modulation of CD4+CD25high regulatory T lymphocytes in multiple sclerosis patients during interferon-β therapy. NeuroImmunoModulation 2012, 19: 283-292.

  10. Silvia Capellino, Marco Cosentino, Wolff C, Schmidt M, Grifka J, Rainer H. Straub. Catecholamine-producing cells in the synovial tissue during arthritis: modulation of sympathetic neurotransmitters as new therapeutic target. Annals of the Rheumatic Diseases 2010, 69: 1853-1860.

D’Elios Mario Milco

Head, Nanomedicine & Immunology Unit

Unità di Nanomedicina & Immunologia
Dipartimento di Medicina Sperimentale e Clinica

Università di Firenze

Largo Brambilla, 3
50134 Firenze – Italia
Tel. 055 275 8331
Fax. 055 427 1494

Collaboratori
  • Marisa Benagiano, Nano Immunologist
  • Chiara Della Bella, Cellular immunologist
  • Nagaja Capitani, Molecular biologist
  • Alessia Grassi, Cellular biologist
  • Sara Pratesi, Molecular immunologist
  • Katarzyna Hołownia, Clinical immunologist

Keywords su ambiti di competenza:
Immune responses in infectious diseases, nanomedicine, vaccines, autoimmunity, atherosclerosis, microbiome, primary immunodeficiency.

 

Tecniche utilizzate:

Cytokine detection, nanotechnology, FACS analysis, ELISPOT, T-cell cloning, B cells, cell biology, polymerase chain reaction, mass spectrometry.

Farina Cinthia

Head, Immunobiology of Neurological Disorders Unit

Divisione di Neuroscienze

 Istituto di Neurologia Sperimentale
Ospedale San Raffaele

Via Olgettina, 58
20132 Milano – Italia
Tel. 02 264 361 80

Collaboratori
  • Emanuela Colombo, biotechnologist, post-doc
  • Marco Di Dario, biologist, Specialized in Pathology
  • Sundararajan Srinivasan, bioinformatician, PhD student
  • Alessandra Russo, Biologist, fellow
  • Anthea De Angelis, biologist, fellow
  • Daniela Triolo, PhD, Technician

Keywords su ambiti di competenza:
Multiple Sclerosis and EAE, glia cell biology, neurodegeneration, innate and adaptive immunity, bioinformatics and systems biology, neurotrophic factors, skeletal muscle.

 

Tecniche utilizzate:

Molecular and cellular biology of glial/neuronal/immune cells. Functional genomics and bioinformatics. Histology of lymphoid, skeletal and nervous tissue. Experimental mouse models.

Recent Publications:
  1. Emanuela Colombo, Stefania Romaggi, Marina Mora, Lucia Morandi and Cinthia Farina. A role for inflammatory mediators in the modulation of the neurotrophin receptor p75NTR on human muscle precursor cells. J. Neuroimmunol. 243, 2012.

  2. Emanuela Colombo, Chiara Cordiglieri, Giorgia Melli, Jia Newcombe, Markus Krumbholz, Luis Parada, Enzo Medico, Reinhard Hohlfeld, Edgar Meinl and Cinthia Farina. Stimulation of the neurotrophin receptor TrkB on astrocytes drives nitric oxide production and neurodegeneration. J.Exp. Med. 209, 2012.

  3. Ramesh Menon, Marco Di Dario, Chiara Cordiglieri, Silvia Musio, Loredana La Mantia, Clara Milanese, Anna Luisa Di Stefano, Massimo Crabbio, Diego Franciotta, Roberto Bergamaschi, Rosetta Pedotti, Enzo Medico and Cinthia Farina. Gender-based blood transcriptomes and interactomes in multiple sclerosis: Involvement of SP1 dependent gene transcription. J. Autoimmunity 38, 2012.

  4. Emanuela Colombo, Stefania Romaggi, Flavia Blasevich, Marina Mora, Chiara Falcone, Hanns Lochmüller, Lucia Morandi and Cinthia Farina. The neurotrophin receptor p75NTR is induced on mature myofibers in inflammatory myopathies and promotes myotube survival to inflammatory stress. Neuropath. Appl. Neurobiol. 38, 2012.

  5. Emanuela Colombo, Francesco Bedogni, Isabella Lorenzetti, Nicoletta Landsberger, Stefano C. Previtali and Cinthia Farina. Autocrine and immune cell derived BDNF in human skeletal muscle: implications for myogenesis and tissue regeneration. J. Pathology 231 (2), 2013.
  6. Emanuela Colombo, Paolo Tentorio, Silvia Musio, Klaus Rajewsky, Rosetta Pedotti, Stefano Casola and Cinthia Farina. Skewed B cell differentiation affects lymphoid organogenesis but not T-cell mediated autoimmunity. Clin Exp Immunol. 176, 2014.

  7. Emanuela Colombo, Marco Di Dario, Eleonora Capitolo, Linda Chaabane, Jia Newcombe, Gianvito Martino and Cinthia Farina. Fingolimod may support neuroprotection via blockade of astrocyte nitric oxide. Annals Neurol. 2014.

Furlan Roberto

Head, Clinical Neuroimmunology Unit

Unità di Neuroimmunologia Clinica

 Istituto di Neurologia Sperimentale
Divisione di Neuroscienze
Istituto Scientifico San Raffaele

Via Olgettina, 60
20132 Milano – Italia
Tel. 02 26434867
Fax. 02 26434855

Collaboratori
  • Annamaria Finardi, tecnico
  • Annamaria Nigro, Post-Doc
  • Giacomo Casella, dottorando
  • Federico Colombo, dottorando
  • Tommaso Croese, specializzando

Keywords su ambiti di competenza:
EAE, gene therapy, microvesicles, psychiatric disorders.

 

Tecniche utilizzate:

Tissue culture, molecular biology, EAE, biochemistry, neuropathology, immunology, cell biology.

Grimaldi Luigi

Primario

U.O.C. Neurologia e Centro Regionale Hub Sclerosi Multipla

 Istituto “G. Giglio” di Cefalù (PA)

Contrada Pietrapollastra s.n.c.
90015 Cefalù (PA) – Italia
Tel. 0921 920340
Fax. 0921 920405

Collaboratori
  • Gaetano VItello, aiuto
  • Graziella Callari, aiuto
  • Barbara Palmeri, aiuto
  • Francesca Ruscica, aiuto
  • Maria Andriolo, biologa

Keywords su ambiti di competenza:
Epidemiologia, trials clinici.

Pubblicazioni U.O.C. Neurologia – Cefalù (2011 – 2015)
  1. Ghezzi A, Grimaldi LME, Marrosu MG, Pozzilli C, Comi G, Bertolotto A, Troiano M, Gallo P, Capra R, Centonze D, Millefiorini E, Sotgiu S, Brescia Morra V, Amato MP, Lugaresi L, Mancardi G, Caputo D, Montanari E, Provinciali L, Durelli L, Bergamaschi R, Bellantonio P, Tola MR, Cottone A, Savettieri L, Tedeschi G e Gruppo di Studio SM-SIN. Natalizumab therapy of multiple sclerosis: recommendations of the Multiple Sclerosis Study Group – Italian Neurological Society. Neurol. Sci. 2011;32:351-358 (I.F.: 1.495)

  2. Mancardi GL, Tedeschi G, Amato MP, D’Alessandro R, Drago F, Milanese C, Popoli P, Rossi P, Savettieri G, Tola MR, Comi G, Pozzilli C, Bertolotto A, Marrosu MG, Grimaldi LME, Laroni A, Vanacore N, Covezzoli A, De Rosa M, Piccinni C, Montanaro N, Periotto L, Iommelli R, Tomino C, Provinciali L. Three years of experience: the Italian registry and safety data update. Neurol. Sci. 2011;31:S295-S297 (I.F.: 1.495)

  3. Sangalli F, Moiola L, Bucello S, Annovazzi P, Rizzo A, Radaelli M, Vitello G, Grimaldi LME, Ghezzi A, Martinelli V, Comi G. Efficacy and tolerability of natalizumab in relapsing-remitting multiple sclerosis patients: a post-marketing observational study. Neurol. Sci. 2011;31:S299-S302 (I.F.: 1.495)

  4. Smolders J, Hupperts R, Barkhof F, Grimaldi LME, Holmoy T, Killestein J, Rieckmann P, Schluep M, Vieth R, Hostalek U, Ghazi-Visser L, Beelke M, on behalf of the SOLAR study group. Efficacy of Vitamin D3 as Add-on Therapy in Patients with Relapsing–Remitting Multiple Sclerosis Receiving Subcutaneous Interferon Beta-1a: A Phase II, Multicentre, Double-blind, Randomized, Placebo-Controlled Trial. J. Neurol Sci. 2011;311:44-49. doi: 10.1016/j.jns.2011.04.013. Epub 2011 May 28 (I.F.: 2.243)

  5. Patti F, Amato MP, Trojano M, Bastianello S, Tola MR, Picconi O, Cilia S, Cottone S, Centonze D, Gasperini C, on behalf of the COGIMUS Study Group. Quality of life, depression and fatigue in mildly disabled patients with relapsing-remitting multiple sclerosis receiving subcutaneous interferon beta-1a: 3-year results from the COGIMUS (COGnitive Impairment in MUltiple Sclerosis) study. Mult. Scler. 2011;17:991-1001. doi: 10.1177/1352458511401943. Epub 2011 Apr 18 (I.F.: 4. 472)

  6. Martinelli V, Cocco E, Capra R, Salemi G, Gallo P, Capobianco M, Pesci I, Ghezzi A, Pozzilli C, Lugaresi A, Bellantonio P, Amato MP, Grimaldi LM, Trojano M, Mancardi GL, Bergamaschi R, Gasperini C, Rodegher M, Straffi L, Ponzio M, Comi G, for The Italian Mitoxantrone Group. Acute myeloid leukemia in italian patients multiple with sclerosis treated with mitoxantrone. Neurology 2011;77:1887-1895. doi: 10.1212/WNL.0b013e318238ee00. Epub 2011 Nov 9 (I.F.: 8.303)

  7. Bastianello S, Giugni E, Amato MP, Tola MR, Trojano M, Galletti S, Luccichenti G, Quarantelli M, Picconi O, Patti F, on behalf of the COGIMUS study group. Changes in magnetic resonance imaging disease measures over 3 years in mildly disabled patients with relapsing-remitting multiple sclerosis receiving interferon β-1a in the COGnitive Impairment in MUltiple Sclerosis (COGIMUS) study. BMC Neurol. 2011;11:125. doi: 10.1186/1471-2377-11-125 (I.F.: 2.486)

  8. Patti F, Amato MP, Trojano M, Bastianello S, Tola MR, Picconi O, Cilia S, Cottone S and Grimaldi LME; on behalf of the COGIMUS study group. Longitudinal changes in social functioning in mildly disabled patients with relapsing–remitting multiple sclerosis receiving subcutaneous interferon beta-1a: results from the COGIMUS (COGnitive Impairment in MUltiple Sclerosis) study (II). Qual Life Res. 2012 Sep;21(7):1111-21. doi: 10.1007/s11136-011-0021-6. Epub 2011 Sep 28 (I.F.: 2.864)

  9. Marino Gammazza A, Bucchieri F, Grimaldi LME, Conway de Macario E, JL Macario AJL, Zummo F, Cappello F. The molecular anatomy of human Hsp60 and its similarity with that of bacterial orthologus and acetylcholine receptor reveal a potential pathogenetic role of anti-chaperonin immunity in myasthenia gravis. Cell. Mol. Neurobiol. 2012;32:943-947. doi: 10.1007/s10571-011-9789-8. Epub 2012 Jan 19 (I.F.: 2.201)

  10. Grimaldi L, Barkhof F, Beelke M, Burton J, Holmoy T, Hupperts R, Killestein J, Rieckmann P, Schluep M, Smolders J; on behalf of the SOLAR study group. A randomized trial of high-dose vitamin D2 in relapsing-remitting multiple sclerosis. Neurology 2012;78:841. doi: 10.1212/01.wnl.0000413180.13413.ce (I.F.: 8.303)

  11. Grimaldi LME, Prosperini L, Vitello G, Borriello G, Fubelli F, Pozzilli C. MRI-based analysis of natalizumab therapeutic window in multiple sclerosis. Mult. Scler. 2012;18:1337-9 doi: 10.1177/1352458512439438. Epub 2012 Mar 2 (I.F.: 4.472)

  12. Laroni A, Giacomazzi CG, Grimaldi LME, Gallo P, Sormani MP, Bertolotto A, McDermott JL, Gandoglia I, Martini I, Vitello G, Rinaldi F, Barzon L, Militello V, Pizzorno M, Bandini F, Capello E, Palù G, Uccelli A, Mancardi GL, Varnier OE. Urinary JCV-DNA testing during natalizumab treatment may increase accuracy of PML risk stratification. J. Neuroimmune Pharmacol. 2012;7:665-672. doi: 10.1007/s11481-012-9366-z. Epub 2012 May 16 (I.F.: 3.172)

  13. Ghezzi A, Annovazzi P, Cocco E, Coarelli G, Lugaresi A, Rovaris M, Patti F, Capello E, Rodegher ME, Moiola L, Malucchi S, Salemi G, De Rossi N, Provinciali L, Perini P, Bergamaschi R, Scarpini E, Lus G, Gallo A, Tola MR, Amato MP, Rottoli MR, Bianchi A, Comi G and the MS Study Group-Italian Society of Neurology. Endovascular treatment of CCSVI in patients with multiple sclerosis: clinical outcome of 462 cases. Neurol. Sci. 2013;9:1633-7. doi: 10.1007/s10072-013-1300-5. Epub 2013 Jan 25. (I.F.: 1.495)

  14. Ghezzi A, Annovazzi P, Amato MP, Capello E, Cavalla P, Cocco E, Falcini M, Gallo A, Patti F, Perini P, Rodegher ME, Rovaris M, Rottoli MR, Comi G and the MS Study Group-Italian Society of Neurology. Adverse events after endovascular treatment of chronic cerebro-spinal venous insufficiency (CCSVI) in patients with multiple sclerosis. Mult Scler. 2013;7:961-3. doi: 10.1177/1352458513475491. Epub 2013 Feb 4 (I.F.: 4.472)

  15. Ghezzi A, Pozzilli C, Grimaldi LME, Moiola L, Brescia Morra V, Lugaresi A, Lus G, Rinaldi F, Rocca MA, Trojano M, Bianchi A, Comi G, Filippi M and the Italian MS Study Group. Natalizumab in pediatric multiple sclerosis: results of a cohort of 55 cases. Mult. Scler. 2013;8:1106-12. doi: 10.1177/1352458512471878. Epub 2013 Feb 11. (I.F.: 4.472)

  16. Patti F, Morra VB, Amato MP, Trojano M, Bastianello S, Tola MR, Cottone S, Plant A, Picconi O; COGIMUS Study Group. Subcutaneous interferon β-1a may protect against cognitive impairment in patients with relapsing-remitting multiple sclerosis: 5-year follow-up of the COGIMUS study. PLoS One. 2013 Aug 30;8(8):e74111. doi: 10.1371/journal.pone.0074111. eCollection 2013 (I.F.: 3.534)

  17. Comi G, Battaglia MA, Bertolotto A, Del Sette M, Ghezzi A, Malferrari G, Salvetti M, Sormani MP, Tesio L, Stolz E, Zaratin P, Mancardi G and the CoSMo Collaborative Study Group. Observational case-control study of the prevalence of chronic cerebrospinal venous insufficiency in multiple sclerosis: results from the CoSMo study. Mult. Scler. 2013;11:1508-17. doi: 10.1177/1352458513501231. Epub 2013 Sep 6 PMID: 24126064 (I.F.: 4.472)

  18. Sorosina M, Brambilla P, Clarelli F, Barizzone N, Lupoli S, Guaschino C, Osiceanu AM, Moiola L, Ghezzi A, Coniglio G, Patti F, Mancardi G, Manunta P, Glorioso N, Guerini FR, Bergamaschi R, Perla F; PROGRESSO; PROGEMUS, Martinelli V, Cusi D, Leone M, Comi G, D’Alfonso S, Martinelli-Boneschi F. Genetic burden of common variants in progressive and bout-onset multiple sclerosis. Mult. Scler. 2013 Nov 28. [Epub ahead of print] PMID: 24277324 (I.F.: 4.472)

  19. Bertolotto A, Capobianco M, Amato MP, Capello E, Capra R, Centonze D, Di Ioia M, Gallo A, Grimaldi L, Imberti L, Lugaresi A, Mancinelli C, Marrosu MG, Moiola L, Montanari E, Romano S, Musu L, Paolicelli D, Patti F, Pozzilli C, Rossi S, Salvetti M, Tedeschi G, Tola MR, Troiano M, Zaffaroni M, Malucchi S. Guidelines on the clinical use of the detection of neutralizing antibodies (NAbs) to IFN beta in Multiple Sclerosis therapy: report of the Italian Multiple Sclerosis Study group. Neurol Sci. 2014;35:307-16 doi: 10.1007/s10072-013-1616-1. Epub 2013 Dec 29. PMID: 24374787 (I.F.: 1.495)

  20. Vermersch P, Czlonkowska A, Grimaldi LM, Confavreux C, Comi G, Kappos L, Olsson T, Benamor M, Bauer D, Truffinet P, Church M, Miller AE, Wolinsky JS, Freedman MS, and O’Connor P; for the TENERE Trial Group. Teriflunomide versus subcutaneous interferon beta-1a in patients with relapsing multiple sclerosis: a randomised, controlled phase 3 trial. Mult. Scler. 2014;20:705-16. doi: 10.1177/1352458513507821. Epub 2013 Oct 14. PMID: 24126064 (I.F.: 4.472)

  21. Bertolotto A, Capobianco M, Amato MP, Capello E, Capra R, Centonze D, Di Ioia M, Gallo A, Grimaldi L, Imberti L, Lugaresi A, Mancinelli C, Marrosu MG, Moiola L, Montanari E, Romano S, Musu L, Paolicelli D, Patti F, Pozzilli C, Rossi S, Salvetti M, Tedeschi G, Tola MR, Troiano M, Zaffaroni M, Malucchi S. Erratum to: Guidelines on the clinical use of the detection of neutralizing antibodies (NAbs) to IFN beta in Multiple Sclerosis therapy: report of the Italian Multiple Sclerosis Study group. Neurol. Sci. 2014 Feb 2; PMID: 24487700 (I.F.: 1.410)

  22. Guaschino C, Esposito F, Liberatore G, Colombo B, Annovazzi P, D’Amico E, Cavalla P, Capello E, Capra R, Galimberti D, Tedeschi G, Grimaldi L, PROGRESSO Group, PROGEMUS GROUP, Leone M, D’Alfonso S, Martinelli V, Comi G, Martinelli-Boneschi F. Familial clustering in Italian progressive-onset and bout-onset multiple sclerosis. Neurol. Sci. 2014;35:789-91 doi: 10.1007/s10072-014-1650-7. Epub 2014 Feb 11 PMID: 24514917 (I.F.: 1.410)

  23. Grimaldi LME, Zappalà G, Castellano A, Iemolo F, Ruggieri S, Bruno G, Paolillo A. A pilot study on the use of interferon beta-1a in early Alzheimer’s disease subjects. J. Neuroinflammation 2014 Feb 13; doi:10.1186/1742-2094-11-30; PMID: 24524367 (I.F.: 4.351)

  24. Bravatà V, 
Minafra L, 
Callari G, 
Gelfi C, Grimaldi LM. Analysis of thiamine transporter genes in sporadic beriberi: a case report. Nutrition. 2014 Apr 30(4):485-8. doi: 10.1016/j.nut.2013.10.008. Epub 2013 Oct 19. PMID: 24607307 (I.F.: 2.859)

  25. Tamburo E, Varrica D, Dongarrà G, Grimaldi LME. Trace Elements in scalp hair samples from patients with relapsing-remitting multiple sclerosis. PLoS One. 2015 Apr 9;10(4):e0122142. doi: 10.1371/journal.pone.0122142. eCollection 2015. PMID: 25856388 [PubMed – in process] PMCID: PMC4391939 (I.F.: 3.534)

  26. Barizzone N, Zara I, Sorosina M, Lupoli S, Porcu E, Pitzalis M, Zoledziewska M, Esposito F, Leone M, Mulas A, Cocco E, Ferrigno P, Guerini FR, Brambilla P, Farina G, Murru R, Deidda F, Sanna S, Loi A, Barlassina C, Vecchio D, Zauli A, Clarelli F, Braga D, Poddie F, Cantello R, Martinelli V, Comi G, Frau J, Lorefice L, Pugliatti M, Rosati G, the PROGEMUS Group, the PROGRESSO Group, Melis M, Marrosu MG, Cusi D, Cucca F, Martinelli Boneschi F, Sanna S, D’Alfonso S. The burden of Multiple Sclerosis variants in continental Italians and Sardinians. Mult. Scler. 2015. (I.F.: 4.472) In press

Lolli Francesco

Associate professor Biomedical Techniques

SOD Neurofisiopatologia
Dipartimento di Scienze Biomediche Sperimentali e Cliniche

 Università degli Studi di Firenze

Viale Pieraccini, 6
50127 Firenze – Italia
Tel. 055 275 8420
Fax. 055 275 8411

Collaboratori
  • Paolo Rovero, Dipartimento NeuroFarBa, Sezione Scienze Farmaceutiche, Unità PeptLab, Università degli Studi di Firenze
  • Anna Maria Papini, Dipartimento di Chimica, Unità PeptLab, Università degli Studi di Firenze

Keywords su ambiti di competenza:
B-cell responses in neuroimmunological diseases.

 

Tecniche utilizzate:
Elisa, peptides, B-cells methods

Martino Gianvito

Head, Neuroimmunology Unit

Unità di Neuroimmunologia

 Ospedale San Raffaele
Divisione di Neuroscienze
INSpe (Ist. Neurologia Sperimentale)

Via Olgettina, 58 – V° Piano
Palazzo San Gabriele 1
20132 Milano – Italia
Tel. 02 264 349 58
Fax. 02 264 348 55

Collaboratori
  • Marco Bacigaluppi, Ricercatore
  • Luca Muzio, Ricercatore
  • Paola Panina, Ricercatore
  • Martina Absinta, Ricercatore
  • Perego Jessica, Ricercatrice
  • Semerano Aurora, Medico Ricercatore
  • Erica Butti, Post-Doc
  • Fagiani Francesca, Post-Doc
  • Mariotti Valeria, Post-Doc
  • Murtaj Valentina, Post-Doc
  • Peri Carolina, Post-Doc
  • Viotti Alice, Post-Doc
  • Andrea Bergamaschi, Tecnico di Laboratorio
  • Elena Brambilla, Tecnico di Laboratorio
  • Francesca Ruffini, Tecnico di Laboratorio
  • Romeo Valentina, Tecnico di Laboratorio
  • Sabovic Iva, Tecnico di Laboratorio
  • Pedrini Edoardo, Bioinformatico
  • Bezukladova Svetlana, studente di dottorato
  • Genchi Angela, studente di dottorato
  • Bonazza Gino, tirocinante post-laurea
  • Dell’Acqua Beatrice, tirocinante post-laurea
  • Giglione Claudia, tirocinante pre-laurea
  • Groppuso Serena, tirocinante pre-laurea

Keywords su ambiti di competenza:
Sclerosi, cellule staminali neurali, sistema nervoso centrale, microglia, infiammazione, encefalite autoimmune sperimentale, cellule staminali pluripotenti, ischemia cerebrale, neuroprotezione, modello di trauma del midollo spinale.

 

Tecniche utilizzate:
Colture cellulari, rt-PCR, microcopia confocale, istologia, patch clamp, microdissezione, analisi di espressione genica, encefalite autoimmune sperimentale, ischemia cerebrale sperimentale, modello di trauma del midollo spinale, modelli transgenici.

Pubblicazioni
  1. PLUCHINO S, QUATTRINI A, BRAMBILLA E, GRITTI A, SALANI G, DINA G, GALLI R, BERGAMI A, FURLAN R, DELCARRO U, AMADIO S, COMI G, VESCOVI AL, MARTINO G. Injection of adult neurospheres induce clinical recovery in a chronic model of multiple sclerosis. Nature 2003;422:688-694.

  2. FURLAN R, BERGAMI A, CANTARELLA D, BRAMBILLA E, TANIGUCHI M, DELLABONA P, CASORATI G, MARTINO G. Activation of invariant NKT cells by a-GalCer administration protects mice from MOG35-55-induced EAE: a critical role for administration route and IFNg. Eur. J. Immunol. 2003:33:1830-1838.

  3. LAMPASONA V, FRANCIOTTA DM, FURLAN R, ZANABONI S, FAZIO R, BONIFACIO E, COMI G, MARTINO G. Similar frequency of anti-MOG IgG and IgM in MS patients and healthy subjects. Neurology 2004;62:2092-2094.

  4. PLUCHINO S, ZANOTTI L, ROSSI B, BRAMBILLA E, OTTOBONI L, SALANI G, MARTINELLO M, CATTALINI A, BERGAMI A, FURLAN R, COMI G, CONSTANTIN G, MARTINO G. Neurosphere-derived multipotent precursors promote neuroprotection by an immunomodulatory mechanism. Nature 2005;436:266-271.

  5. MARTINO G, PLUCHINO S. The therapeutic potential of neural stem cells. Nat. Rev. Neurosci. 2006;7:395-406.

  6. FURLAN R, BERGAMI A, BRAMBILLA E, BUTTI E, DE SIMONI MG, CAMPAGNOLI M, MARCONI P, COMI G, MARTINO G. HSV-1-mediated IL-1 receptor antagonist gene therapy ameliorates MOG35-55 induced experimental autoimmune encephalomyelitis in C57BL/6 mice. Gene Ther. 2007;14:93-98 (Epub ahead of print, August 24, 2006)

  7. PLUCHINO S, MUZIO L, IMITOLA J, DELEIDI M, ALFARO-CERVELLO C, SALANI G, PORCHERI C, BRAMBILLA E, BERGAMASCHI A, GARCIA-VERDUGO JM, COMI G, KHOURY SJ*, MARTINO G*. Chronic inflammation induces a non cell-autonomous dysfunction of the endogenous brain stem cell compartment. (*equally contributed) Brain 2008;131: 2564-2578 [Epub ahead of print on Aug 30, 2008].

  8. CENTONE D*, MUZIO L*, ROSSI S, CAVASINNI F, DE CHIARA V, BERGAMI A, MUSELLA A, MARCELLO D’AMELIO M, CAVALLUCCI V, MARTORANA A, BERGAMASCHI A, CONCIONI MT, DIAMANTINI A, BUTTI E, COMI G, BERNARDI G, CECCONI F, BATTISTINI L, FURLAN R, MARTINO G. Inflammation triggers synaptic alteration and degeneration in experimental autoimmune encephalomyelitis. J. Neurosci. 2009;29:3442-3452 (*equally contributed)

  9. PLUCHINO S, ZANOTTI L, BRAMBILLA E, ROVERE-QUERINI P, CAPOBIANCO A, ALFARO-CERVELLO C, SALANI G, BORSELLINO G, BATTISTINI L, PONZONI M, DOGLIONI C, GARCIA-VERDUGO JM, COMI G, MANFREDI AA, MARTINO G. Multipotent neural stem/precursor cells protects from central nervous system autoimmunity by restraining dendritic cell function. PLoS One 2009;4:e5959 [Epub ahead of print on Jun 19, 2009]

  10. PLUCHINO S*, GRITTI A*, BLAZER E, AMADIO S, BRAMBILLA E, BORSELLINO G, COSSETTI C, DEL CARRO U, COMI G, ‘T HART B, VESCOVI A, MARTINO G. Human neural stem cells ameliorate autoimmune encephalomyelitis in non human primates. Ann Neurol. 2009;66:343-354 (*equally contributed)

  11. MARTINO G, FRANKLIN RJ, VAN EVERCOOREN AB, KERR DA, the Stem Cells in Multiple Sclerosis (STEMS) Consensus Group. Stem cell transplantation in multiple sclerosis: current status and future prospects. Nat Rev Neurol, 2010;6:247–255 [Epub ahead of print on Apr 20, 2010].

  12. CENTONZE D, MUZIO L, ROSSI S, FURLAN R, BERNARDI G, MARTINO G. The link among inflammation, synaptic transmission and neurodegeneration in multiple sclerosis. Cell Death Differ. 2010;17:1083-1091 [Epub ahead of print on Nov 20, 2009].

  13. MARTINO G, PLUCHINO S, BONFANTI L, SCHWARTZ M. Brain regeneration in physiology and pathology: the immune signature driving therapeutic plasticity of neural stem cells. Physiol. Rev. 2011;91:1281-1304.

  14. DEFEO D, MERLINI A, LATERZA C, MARTINO G. Neural stem cell transplantation in CNS disorder: from cell replacement to neuroprotection. Curr. Opin. Neurol. 2012;25:322–333.

  15. KOKAIA Z*, MARTINO G*, SCHWARTZ M*, LINDVALL O. Cross-talk between neural stem cells and immune cells: the key to better brain repair? Nat. Neurosci. 2012;15:1078-1087. (*equally contributed)

  16. BUTTI E*, BACIGALUPPI M*, ROSSI S, CAMBIAGHI M, BARI M, CEBRIAN SILLA A, BRAMBILLA E, MUSELLA A, DE CEGLIA R, TENEUD L, DE CHIARA V, D’ADAMO P, GARCIA-VERDUGO JM, COMI G, MUZIO L, QUATTRINI A, LEOCANI L, MACCARRONE M, CENTONZE D, MARTINO G. Subventricular zone neural progenitors protect striatal neurons from glutamatergic excitoxicity. Brain 2012;115:3320-3335 [Epub ahead of print on Sep. 24, 2012]. (*equally contributed)

  17. LATERZA C, MERLINI A, DE FEO D, RUFFINI F, MENON R, ONORATI M, FREDRICKX E, MUZIO L, LOMBARDO A, COMI G, QUATTRINI A, TAVEGGI AC, FARINA C, CATTANEO E, MARTINO G. iPSC-derived neural precursors exert a neuroprotective role in immune-mediated demyelination via the secretion of leukemia inhibitory factor. Nat. Commun. 2013;4:2597.

  18. SANDRONE A, BACIGALUPPI M, GALLONI MR, CAPPA SF, MORO A, CATANI M, FILIPPI M, MONTI MM, PERANI D*, MARTINO G*. Weighing brain activity with the balance: Angelo Mosso’s original manuscripts come to light. Brain 2014;137:621-633 (*equally contributed).

  19. MARTINO G, BUTTI E, BACIGALUPPI M. Neurogenesis or non-neurogenesis: this is the question. J. Clin. Invest. 2014;124:970-973.

  20. ONORATI M*, CASTIGLIONI V*, BIASCI D**, CESANA E**, MENON R**, VUONO R, TALPO F, GOYA RL, LYONS P, BULFAMANTE GP, MUZIO L, MARTINO G, TOSELLI M, FARINA C, BARKER RA, BIELLA G, CATTANEO E. Molecular and functional definition of the developing human striatum. Nat. Neurosci. 2014;17:1804-1815 (*,**equally contributed).

  21. ARNÒ B, GRASSIVARO F, ROSSI C, BERGAMASCHI A, GRETER M, FAVARO R, COMI G, BECHER B, MARTINO G*, MUZIO L*. Neural progenitors cells orchestrate microglia migration and positioning into the developing cortex. Nat. Comm. 2014;5:5611 (*equally contributed).

  22. MOZAFARI S, LATERZA C, ROUSSEL D, BACHELIN C, MARTEYN A, DEBOUX C, MARTINO G, BARON-VAN EVERCOOREN A. Skin-derived neural precursors competitively generate functional myelin in adult demyelinated mice. J. Clin. Inv. 2015;125:3642-3656.

  23. BACIGALUPPI M, RUSSO G*, PERUZZOTTI-JAMETTI L*, ROSSI S, SANDRONE S, GALLIZIOLI M, DE CEGLIA R, BUTTI E, STUDER V, COLOMBO E, REITMEIR R, MOTTA C, MUZIO L, COMI G, FARINA C, LETTERIO POLITI LS, INVERNIZZI RW, HERMANN DM*, CENTONZE D*, MARTINO G. Neural stem cell transplantation promotes post-ischemic neuronal plasticity by regulating the expression of glutamate transporters. J. Neurosci. 2016;36:10529-10544 (*equally contributed).

  24. DE FEO D, MERLINI A, BRAMBILLA E, OTTOBONI L, LATERZA C, MENON R, SRINIVASAN S, FARINA C, MANTEIGA JMG, BUTTI E, BACIGALUPPI M, COMI G, GRETER M, MARTINO G. Neural precursor cell-secreted TGF-β2 redirects inflammatory monocyte-derived cells in CNS autoimmunity. J. Clin. Inv. 2017;127:3937-3953 [Epub ahead of print on September 25, 2017]

  25. ROSSI C, CUSIMANO M, ZAMBITO M, FINARDI A, CAPOTONDO A, GARCIA-MANTEIGA JM, COMI G, FURLAN R, MARTINO G*, MUZIO L*. IL-4 modulates microglia homeostasis and attenuates the early slowly progressive phase of Amyotrophic Lateral Sclerosis. Cell Death Dis. 2018;9:250 (*equally contributed).

  26. CUSIMANO M, BRAMBILLA E, CAPOTONDO A, DE FEO D, TOMASSO A, COMI G, MUZIO L*, MARTINO G*. Selective killing of spinal cord neural stem cells impairs locomotor recovery in a mouse model of spinal cord injury. J. Neuroinflamm. 2018;15:58 (*equally contributed).

  27. BECHER J, SIMULA L, VOLPE E, PROCACCINI C, LA ROCCA C, D’ACUNZO P, CIANFANELLI V, STRAPPAZZON F, CARUANA I, NAZIO F, WEBER G, GIGANTINO V, BOTTI G, CICCOSANTI F, BORSELLINO G, CAMPELLO S, MANDOLESI G, DE BARDI M, FIMIA GM, D’AMELIO M, RUFFINI F, FURLAN R, CENTONZE D, MARTINO G, BRAGHETTA P, CHRISAM M, BONALDO P, MATARESE G, LOCATELLI F, BATTISTINI L, CECCONI F. AMBRA1 controls regulatory T-cell differentiation and homeostasis upstream of the FOXO3-FOXP3 axis. Dev. Cell 2018;47:592-607.

  28. CORADA M, ORSENIGO F, PARAMESHWAR BHAT G, CONZE LL, BREVIARIO F, CUNHA SI, CLAESSON-WELSH L, BEZNOUSSENKO GV, MIRONOV AA, BACIGALUPPI M, MARTINO G, PITULESCU ME, ADAMS RH, MAGNUSSON P, DEJANA E. Fine tuning of Sox17 and canonical Wnt coordinates the permeability properties of the blood-brain barrier. Circ. Res. 2019;124:511-525.

  29. BUTTI E, BACIGALUPPI M, CHAABANE L, RUFFINI F, BRAMBILLA E, BERERA G, MONTONATI C, QUATTRINI A, MARTINO G.Neural stem cells of the subventricular zone contribute to neuroprotection of the corpus callosum after cuprizone-induced demyelination. J. Neurosci. 2019;39:5481-5492 [Epub ahead of print on May 28, 2019].

  30. GRASSIVARO F, MENON R, ACQUAVIVA M, OTTOBONI L, RUFFINI F, BERGAMASCHI A, MUZIO M, FARINA C*, MARTINO G*. Convergence between microglia and peripheral macrophages phenotypes during development and neuroinflammation. J. Neurosci. 2020;40 (4):784-795 [Epub ahead of print on Dec. 9, 2019] (*co-correspondence)

  31. VIGGIANO D, WAGNER C, MARTINO G, NEDERGAARD M, ZOCCALI C, UNWIN R, CAPASSO G. Mechanisms of cognitive dysfunction in CKD. Nat. Rev. Nephrol. 2020;16:452-469 [Epub ahead of print on March 31]

  32. MUZIO L, SIRTORI R*, GORNATI D*, ELEUTERI S, FOSSAGHI A, MANZONI L, OTTOBONI L, DE FEO L, QUATTRINI A, MASTRANGELO E, SORRENTINO L, SCALONE E, COMI G, RIVA N, MILANI M*, SENECI P*, MARTINO G*. Therapeutic effects of retromer stabilization in amyotrophic lateral sclerosis models. Nat. Comm. 2020;11:3848 (*equally contributed)

  33. STAROST L, LINDNER M, HEROLD M, XU YKT, DREXLER HCA, Heß K, EHRLICH M, OTTOBONI L, STEHLING M, RöPKE A, THOMAS C, SCHöLER HR, ANTEL J, WINKLER J, MARTINO G*, KLOTZ L*, KUHLMANN T*. Extrinsic immune cell-derived, but not intrinsic oligodendroglial factors contribute to oligodendroglial differentiation block in multiple sclerosis. Acta Neuropathol. 2020;10.1007/s00401-020-02217-8 [Epub ahead of print on Spet. 7, 2020]. (*equally contributed)

  34. MOZAFARI S, STAROST L, MANOT B, GARCIA-DIAZ, XU YKT, ROUSSEL D, LEVY MJF, ANTEL JP, MARTINO G, ANGULO MC, KUHLMANN T, BARON-Van EVERCOOREN A. Multiple sclerosis iPS-derived oligodendrocytes conserve their intrinsic properties to functionally interact with axons and glial cells in vivo. Science Advances. 2020;6:eabc6983

  35. MUZIO L, SIRTORI R*, GORNATI D*, ELEUTERI S, FOSSAGHI A, MANZONI L, OTTOBONI L, DE FEO L, QUATTRINI A, MASTRANGELO E, SORRENTINO L, SCALONE E, COMI G, RIVA N, MILANI M*, SENECI P*, MARTINO G*. Therapeutic effects of retromer stabilization in amyotrophic lateral sclerosis models. Nat. Comm. 2020;11:3848 (*equally contributed).

  36. MOZAFARI S, DEBOUX C, LATERZA C, EHRLICH M, KUHLMANN T, MARTINO G, BARON-VAN EVERCOOREN A. Beneficial contribution of iPSC-progeny to connexin 47 dynamics during demyelination-remyelination. Glia 2021;69:1094-1109 [Epub ahead of print on Dec. 10, 2020].

  37. BACIGALUPPI M, SEMERANO A, SALIOU G, SANVITO F, GENCHI A, GULLOTTA G, SALVIONI P, MICHEL P, FILIPPI M, MARTINO G, STRAMBO D, BACIGALUPPI M. Fishing an anemone in the brain: embolized cardiac fibroelastoma revealed after stroke thrombectomy. Eur. Heart J. 2021;ehab019.

  38. TEDESCO M, GIANNESE F, LAZAREVIC D, GIANSANTI V, ROSANO D, MONZANI S, CATALANO I, GRASSI E, ZANELLA E, BOTRUGNO OA, MORELLI L, PANINA BORDIGNON P, CARAVAGNA G, BERTOTTI A, MARTINO G, ALDRIGHETTI L, PASQUALATO S, TRUSOLINO L, CITTARO D*, TONON G*. Chromatin Velocity reveals epigenetic dynamics by single-cell profiling of heterochromatin and euchromatin. Nat. Biotech. [Epub ahead of print on Oct 11, 2021] (*equally contributed).

  39. GENCHI A, SEMEARANO A, SCHWARZ G, DELL’ACQUA B, GULLOTTA GS, SAMPAOLO M, BOERI E, QUATTRINI A, SANVITO F, DIAMANTI S, BERGAMASCHI A, GRASSI S, PODINI P, PANNI P, MICHELOZZI C, SIMONATO F, SCOMAZZI F, REMIDA P, VALVASSORI L, FALINI A, FERRARESE C, MICHEL P, SALIOU G, HAJDU S, BERETTA S, ROVERI L, MASSIMO F, STRAMBO D, MARTINO G, BACIGALUPPI M. Neutrophils predominate the immune signature of cerebral thrombi in COVID-19 stroke patients. Acta Neuropathol. Comm. in press

Matarese Giuseppe

Professor of Immunology & General Pathology

Treg Cell Lab – Dipartimento di Medicina Molecolare e Biotecnologie Mediche (Dipartimento di Eccellenza MUR) & Istituto per l’Endocrinologia e l’Oncologia Sperimentale del Consiglio Nazionale delle Ricerche (IEOS-CNR)

 Università di Napoli “Federico II”

Via S. Pansini, 5
80131 Napoli – Italia
Tel. 081 746 4580
Lab. 081 746 4596
Fax. 081 746 4580

Collaboratori
  • Claudio Procaccini, Ricercatore CNR
  • Fortunata Carbone, Ricercatore CNR
  • Claudia La Rocca, Ricercatore CNR
  • Mario Galgani, Ricercatore Universitario di Tipo B – Unina
  • Paola de Candia, Ricercatore Universitario di Tipo B – Unina
  • Alessandra Colamatteo, Ricercatore Universitario di Tipo A – Unina
  • Sara Bruzzaniti, Assegnista CNR
  • Maria Teresa Lepore, Assegnista CNR
  • Teresa Micillo, Borsista Fondazione S. Lucia
  • Clorinda Fusco, Borsista Fondazione S. Lucia
  • Claudia Russo, Borsista Fondazione S. Lucia
  • Giusy De Rosa, Dottoranda Unina
  • Erica Piemonte, Dottoranda Unina
  • Salvatore De Simone, Tecnico CNR

Keywords su ambiti di competenza:
Sclerosi multipla, EAE, immunotolleranza, Treg cells, metabolismo, immunometabolismo, tessuto adiposo, obesità, leptina.

 

Tecniche utilizzate:
Colture cellulari, EAE, FACS sorting, flussi metabolici (Seahorse analyzer), multicolor FACS analysis, biochimica di base, luminex.

Pubblicazioni (Migliori 10 lavori Gruppo Matarese)
  1. Procaccini C, Garavelli S, Carbone F, Di Silvestre D, La Rocca C, Greco D, Colamatteo A, Lepore MT, Russo C, De Rosa G, Faicchia D, Prattichizzo F, Grossi S, Campomenosi P, Buttari F, Mauri P, Uccelli A, Salvetti M, Brescia Morra V, Vella D, Galgani M, Mottola M, Zuccarelli B, Lanzillo R, Maniscalco GT, Centonze D, de Candia P, Matarese G. Signals of Pseudo-Starvation Unveil the Amino Acid Transporter SLC7A11 as Key Determinant in the Control of Regulatory T Cell Proliferative Potential. Immunity. 2021 Jul 13;54(7):1543-1560.e6. doi: 10.1016/j.immuni.2021.04.014

  2. Li X, Colamatteo A, Kalafati L, Kajikawa T, Wang H, Lim JH, Bdeir K, Chung KJ, Yu X, Fusco C, Porcellini A, De Simone S, Matarese G, Chavakis T, De Rosa V, Hajishengallis G. The DEL-1-β3 integrin axis promotes regulatory T cell responses during inflammation resolution. J Clin Invest. 2020 Aug 20:137530. doi: 10.1172/JCI137530.

  3. Terrazzano G, Bruzzaniti S, Rubino V, Santopaolo M, Matarese G* et al. Type 1 diabetes progression is associated with loss of CD3+CD56+ regulatory T cells that control CD8+ T-cell effector functions. Nat Metab. 2020;2(2):142‐152. doi:10.1038/s42255-020-0173-1 *Corresponding authors.

  4. Procaccini C, Carbone F, Di Silvestre D, Brambilla F, De Rosa V, Galgani M, Faicchia D, Marone G, Tramontano D, Corona M, Alviggi C, Porcellini A, La Cava A, Mauri P, Matarese G. The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements. Immunity 2016 Mar 15;44(3):712.

  5. De Rosa V, Galgani M, Porcellini A, Colamatteo A, Santopaolo M, Zuchegna C, Romano A, De Simone S, Procaccini C, La Rocca C, Carrieri PB, Maniscalco GT, Salvetti M, Buscarinu MC, Franzese A, Mozzillo E, La Cava A, Matarese G. Glycolysis controls the induction of human regulatory T cells by modulating the expression of FOXP3 exon 2 splicing variants. Nat Immunol. 2015 Nov;16(11):1174-84.

  6. Carbone F, De Rosa V, Carrieri PB, Montella S, Bruzzese D, Porcellini A, Procaccini C, La Cava A, Matarese G. Regulatory T cell proliferative potential is impaired in human autoimmune disease. Nat Med. 2014 Jan;20(1):69-74.

  7. Procaccini C, De Rosa V, Galgani M, Abanni L, Calì G, Porcellini A, Carbone F, Fontana S, Horvath TL, La Cava A, Matarese G. An Oscillatory switch in mTOR kinase activity sets regulatory T cell responsiveness. Immunity 2010 Dec14;33(6):929-41.

  8. De Rosa V, Procaccini C, Cali G, Pirozzi G, Fontana S, Zappacosta S, La Cava A, Matarese G. A Key Role of Leptin in the Control of Regulatory T Cell Proliferation. Immunity. 2007;26:241-255.

  9. De Rosa V, Procaccini C, La Cava A, Chieffi P, Nicoletti GF, Fontana F, Zappacosta S, Matarese G. Leptin neutralization interferes with pathogenic T cell autoreactivity in autoimmune encephalomyelitis. J Clin Invest. 2006; 116: 447-455.

  10. Sanna V, Di Giacomo A, La Cava A, Lechler R, Fontana S, Zappacosta S, Matarese G. Leptin surge precedes the onset of autoimmune encephalomyelitis and correlates with the development of pathogenic T cell responses. J Clin Invest. 2003;111:241-50.

Pedotti Rosetta

Head, Experimental Neuroimmunology of CNS diseases

Department of Clinical Neuroscience

Fondazione Istituto Neurologico Carlo Besta

Via Amadeo, 42
20133 Milano – Italia
Tel. 02 23944654

Collaboratori
  • Silvia Musio, Biologist, post-doc
  • Massimo Costanza, biotechnologist, post-doc

Keywords su ambiti di competenza:
Inflammation, central nervous system, neurodegeneration, experimental autoimmune encephalomyelitis, innate and adaptive immunity, type 2 immunity, hormones.

 

Tecniche utilizzate:
Experimental mouse models, gene knockout mouse models, molecular and cellular biology of immune cells, gene expression studies, flow cytometry, pharmacological studies.

Pubblicazioni Rappresentative
  1. Musio S, Costanza M, Poliani L, Fontana E, Cominelli M, Abolafio G, Steinman L, Pedotti R. Treatment with anti-FcεRIα antibody exacerbates EAE and T cell immunity against myelin. Neurology, Neuroimmunology and Neuroinflammation. 4:e342; Published online April 14, 2017 doi:10.1212/NXI.0000000000000342: 2332-7812.

  2. Rigoni R, Fontana E, Guglielmetti S, Fosso B, D’Erchia AM, Maina V, Taverniti V, Castiello MC, Mantero S, Pacchiana G, Musio S, Pedotti R, Selmi C, Mora JR, Pesole G, Vezzoni P, Poliani PL, Grassi F, Villa A, Cassani B. Intestinal microbiota sustain inflammation and autoimmunity induced by hypomorphic RAG defects. J Exp Med. 2016 Mar 7;213(3):355-75. doi: 10.1084/jem.20151116. Epub 2016 Feb 29.

  3. Abou-Hamdan M, Costanza M, Fontana E, Di Dario M, Musio S, Congiu C, Onnis V, Lattanzi R, Radaelli M, Martinelli V, Salvadori S, Negri l, Poliani PL, Farina C, Balboni G, Steinman L, Pedotti R. Critical role for prokineticin 2 in central nervous system autoimmunity. Neurology, Neuroimmunology and Neuroinflammation. 2015 Apr 9;2(3):e95.

  4. Costanza M, Binart N, Steinman L, Pedotti R. Prolactin: A versatile regulator of inflammation and autoimmune pathology. Autoimmunity Reviews 2015 Mar;14(3):223-230.

  5. Menon R, Di Dario M, Cordiglieri C, Musio S, La Mantia L, Milanese C, Di Stefano AL, Crabbio M, Franciotta D, Bergamaschi R, Pedotti R, Medico E, Farina C. Gender-based blood transcriptomes and interactomes in multiple sclerosis: Involvement of SP1 dependent gene transcription. J Autoimmunity. 2012 May;38(2-3):J144-55.

  6. Costanza M, Musio S, Abou-Hamdan M, Binart N, Pedotti R. Prolactin Is Not Required for the Development of Severe Chronic Experimental Autoimmune Encephalomyelitis. J Immunology. 2013 Sep 1;191(5):2082-8.

  7. Piconese S, Costanza M, Musio S, Tripodo C, Poliani PL, Gri G, Burocchi A, Pittoni P, Gorzanelli A, Colombo MP and Pedotti R. Exacerbated experimental autoimmune encephalomyelitis in mast cell-deficient KitW-sh/W-sh mice. Laboratory Investigation 2011 Feb 14;91,627–641.

  8. Piconese S, Gri G, Tripodo C, Musio S, Gorzanelli A, Frossi B, Pedotti R, Pucillo CE, and Colombo MP. Mast cells counteract regulatory T cell suppression through interleukin-6 and OX40/OX40L axis toward Th17 cell differentiation. Blood 2009 Sep 24;114(13):2639-48.

  9. Scabeni S, Lapilla M, Musio S, Gallo B, Ciusani E, Steinman L, Mantegazza R, and Pedotti R. CD4+CD25+ Tregs specific for a thymus-expressed antigen prevent the development of anaphylaxis to self. J Immunology 2008 Apr 1;180(7):4433-40.

  10. Gerdoni E., Gallo B, Casazza S, Musio S, Bonanni I, Pedemonte E, Mantegazza R, Mancardi GL, Pedotti R, Uccelli A. Mesenchymal stem cells effectively modulate immune response against PLP139-151 in experimental autoimmune encephalomyelitis. Annals of Neurology 2007 Mar;61(3):219-27.

  11. Musio S, Gallo B, Scabeni S, Poliani PL, Matarese G, Ohtsu H, Galli SJ, Mantegazza R, Steinman L, Pedotti R. A key regulatory role for histamine in experimental autoimmune encephalomyelitis: Disease exacerbation in histidine decarboxylase-deficient mice. J Immunology. 2006 Jan; 176(1):17-26.

  12. Robinson WH, Fontoura P, Lee BJ, De Vegvar HE, Tom J, Pedotti R, DiGennaro CD, Mitchell DJ, Fong D, Ho PP, Ruiz PJ, Maverakis E, Stevens DB, Bernard CC, Martin R, Kuchroo VK, Van Noort JM, Genain CP, Amor S, Olsson T, Utz PJ, Garren H, Steinman L.Protein microarrays guide tolerizing DNA vaccine treatment of autoimmune encephalomyelitis. Nature Biotechnology. 2003 Sep;21(9):1033-1039.

  13. Pedotti R, DeVoss J, Steinman L, Galli S. Involvement of both ‘allergic’ and ‘autoimmune’ mechanisms in EAE, MS and other autoimmune diseases. Trends Immunology. 2003 Sep;24(9):479-84. With journal cover.

  14. Pedotti R, DeVoss JJ, Youssef S, Mitchell D, Wedemeyer J, Madanat R, Garren H, Fontoura P, Tsai M,  Galli SJ, Sobel RA, Steinman L. Multiple Elements of the Allergic Arm of the Immune Response Modulate Autoimmune Demyelination. Proceedings of the National Academy of Science USA. 2003 Feb 18;100(4):1867-72.

  15. Karpuj MV, Becher MW, Springer JE, Chabas D, Youssef S, Pedotti R, Mitchell D, Steinman L. Prolonged survival and decreased abnormal movements in transgenic model of Huntington disease, with administration of the transglutaminase inhibitor cystamine. Nature Medicine 2002 Feb;8(2):143-9.

  16. Lock C*, Hermans G*, Pedotti R*, Brendolan A, Schadt E, Garren H, Langer-Gould A, Strober S, Cannella B, Allard J, Klonowski P, Austin A, Lad N, Kaminski N, Galli SJ, Oksenberg JR, Raine CS, Heller R, Steinman L. Gene-microarray analysis of multiple sclerosis lesions yields new targets validated in autoimmune encephalomyelitis. Nature Medicine. 2002 May;8(5):500-8. *Equal contribution.

  17. Chabas D, Baranzini SE, Mitchell D, Bernard CCA, Rittling SR, Denhardt DT, Sobel RA, Lock C, Karpuj M, Pedotti R, Heller R,  Oksenberg JR, and Steinman L. “The influence of the proinflammatory cytokine, osteopontine, on autoimmune demyelinating disease”. Science, 2001 Nov, 294, 1731-5.

  18. Pedotti R, Mitchell D, Wedemeyer J,  Karpuj M, Chabas, Hattab E, Tsai M, Galli SJ, Steinman L. “An unexpected version of horror autotoxicus: anaphylactic shock to a self-peptide”. Nature Immunology 2001 Mar;2(3):216-22..

Piccio Laura

Assistant Professor

Brain and Mind Centre
Department of Neurology

 The University of Sydney
Washington University School of Medicine in St. Louis

94 Mallett St Camperdown
NWS 2050, Australia
Tel. +61 2 93510717
Mob. +61 406006813

660 South Euclid, Campus box 8111
63110 St Louis – USA
Tel. (314) 747-4591
Fax (314) 747-1345

Collaboratori
  • Monokesh Sen PhD, postdoctoral fellow
  • Anjie Ge, PhD Student
  • Laura Ghezzii MD, PhD, postdoctoral fellow
  • Luca De Feo, PhD postdoctoral fellow
  • Francesca Pace MD, visiting researcher
  • Robert Mikesell, tecnico di laboratorio
  • Bryan Bollman, tecnico di laboratorio
  • Shin-We Hughes, tecnico di laboratorio
  • Giovanni Pastori, visiting researcher

Keywords su ambiti di competenza:
EAE, MS, microglia, adipochine, dieta, restrizione calorica.

 

Tecniche utilizzate:

Colture cellulari, modelli animali, live imaging, flow cytometry, immunoistochimica.

Pluchino Stefano

Head

Professor of in Regenerative Neuroimmunology and Honorary Consultant in Neurology Dept of Clinical Neurosciences

 University of Cambridge

Clifford Allbutt Building – Cambridge Biosciences Campus
Hills Road, CB2 0HA
Cambridge UK

Collaboratori
  • Regan Hamel Rosetrees Trust PhD student
  • Greg Krzak Clinical Neuroscience PhD student
  • Monica Emili Garcia Segura MRC-DTP PhD candidate
  • Bristena Ionescu MRC-DTP PhD student
  • Alexandra Nicaise ECTRIMS Post doctoral fellow
  • Vasiliki Pappa Laboratory technician
  • Luca Peruzzotti-Jametti Wellcome Trust Clinical Research Career Development fellow
  • Pranathi Prasad MRC-DTP PhD candidate
  • Larissa Roth ERASMUS fellow
  • Cory Willis National MS Society Post doctoral fellow

Keywords su ambiti di competenza:
Advanced therapeutics, Somatic stem cells, Neural stem cells, Oligodendrocyte progenitors, Experimental cell therapies and cell transplantation, CNS regeneration, Mechanisms and molecules of neuroprotection, Extracellular membrane vesicles, including exosomes, and horizontal RNA transfer, Morphogens and morphogenesis, Multiple Sclerosis and experimental autoimmune encephalomyelitis, Nanotechnologies and Synthetic Biology, Experimental stroke, Experimental spinal cord injury, Mobile small ncRNAs, Tissue and cell engineering.

 

Tecniche utilizzate:

Cell culture, Cell transplantation, somatin and induced NSCs, Bioinformatics and Computational Biology, next generation sequencing, Confocaland Super-resolution imaging, Electron microscopy (scanning and transmission), Flow cytometry, Fluorescence microscopy,human iPSCs and human iNSCs, Immunohistochemistry, In situ hybridization, In vitro co-cultures, Magnetic resonance imaging (MRI), Microscopy, Non viral delivery technologies (nanoparticles; liposomes), Reverse engineering, RNA deep sequencing, Semi-quantitative real time RT-PCR, siRNA/shRNA, Unbiased stereology, Viral vector technology for cell engineering and ncRNA delivery.

Ria Francesco

Associate Professor

Department of translational Medicine and Surgery
Section of General Pathology

 Università Cattolica del S. Cuore
IRCCS Fondazione Policlinico Universitario A. Gemelli

L.go F. Vito, 1
00168 Roma – Italia
Tel. 338 466 2776
Fax. 06 3386 446

Collaboratori
  • Maria Tredicine dottoranda

Keywords su ambiti di competenza:
EAE, TCr, TLRs.

 

Tecniche utilizzate:

Colture cellulari, immunoscope, MACS, Citofluorimetria, QRT-PCR.

Salvetti Marco

Neurologo, Responsabile

Centre for Experimental Neurological Therapies (CENTERS)
Dipartimento di Neuroscienze, Salute Mentale e Organi di Renso (NESMOS)

 Università di Roma Sapienza, Ospedale S. Andrea

Via di Grottarossa, 1035 -1039
00189 Roma – Italia
Tel. 06 337 759 94
Fax. 06 337 759 00

Collaboratori
  • Giovanni Ristori, Neurologo, co-responsabile
  • Maria Chiara Buscarinu, Neurologa
  • Giulia Coarelli, Specializzanda in Neurologia
  • Arianna Fornasiero, Neurologa
  • Carlo Mattei, Manager di laboratorio
  • Gianluca Mattei, Biologo
  • Rosella Mechelli, Biologa
  • Silvia Romano, Neurologa
  • Renato Umeton, Bioinformatico
  • Danila Vittori, Specializzanda in Neurologia

Keywords su ambiti di competenza:
Sclerosi multipla, malattie eredodegenerative, Epstein-Barr virus, interazioni geni-ambiente, gene expression noise, neurogenetica, trial clinici, repurposing.

 

Tecniche utilizzate:

Isolamento di tipi cellulari diversi dal circolo periferico (cellule T e B), colture cellulari, estrazione acidi nucleici, real time PCR, digital droplet PCR, genotipizzazione cellulare e virale, deep-sequencing, risonanza magnetica.

Sola Patrizia

Responsabile Centro Malattie demielinizzanti e Laboratorio di Neuroimmunologia

U.O. Neurologia – Dipartimento di Scienze Biomediche
Metaboliche e Neuroscienze
Università di Modena e Reggio Emilia

 Nuovo Ospedale Civile S. Agostino/Estense

Via Giardini 1355
41126 Modena – Italia
Tel. 059 396 1722/2251/2457
Fax 059 296 1323

Collaboratori
  • Dr. Diana Ferraro, Neurologo, Ricercatore a TD
  • Francesca Vitetta, Neurologo, Dirigente Medico a TD
  • Anna Maria Simone, Neurologo, Dottoranda di Ricerca in Neuroscienze
  • Roberta Bedin, Biologa

Keywords su ambiti di competenza:
Bande oligoclonali liquorali, markers biologici.

 

Tecniche utilizzate:

Isoelettrofocusing, immunoblotting, ELISA, PCR

Uccelli Antonio

Associate Professor of Neurology at Department of Neurology, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DINOGMI) Director of Centro di eccellenza per lo studio dei meccanismi molecolari di comunicazione tra cellule: dalla ricerca di base alla clinica (CEBR)

Unità di Neuroimmunologia

 Department of Neurology, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DINOGMI)
Centro di eccellenza per lo studio dei meccanismi molecolari di comunicazione tra cellule: dalla ricerca di base alla clinica (CEBR)

Largo Paolo Daneo, 3
16132 Genova – Italia
Tel. +39 010 353 7028/48
Fax. +39 010 353 8625

Collaboratori
  • Nicole Kerlero De Rosbo, collaboratore
  • Debora Giunti, tecnico
  • Giovanni Novi, specializzando
  • Federico Ivaldi, tecnico
  • Tiziana Vigo, collaboratore
  • Giovanni Ferrara, assegnista
  • Valentina Petrosino, dottoranda
  • Ilaria Gandoglia, specializzanda
  • Benedetta Parodi, borsista
  • Alice Laroni, ricercatrice
  • Consuelo Venturi, collaboratrice
  • Federica Benvenuto, collaboratrice
  • Maria Cellerino, specializzanda
  • Chiara Marini, dottoranda

Keywords su ambiti di competenza:
Chronic and relapsing-remitting EAE, mesenchymal stem cell mode of action, antigen-specific T cells, microglia activation, immunomodulatory miRNA, exosomes, NK regulatory cells.

 

Tecniche utilizzate:

T-cell culture, antigen-specific primary T-cell lines and proliferation assays, NK cell cullture, neural cell culture (astrocytes, microglia, neurons), FACS, electron microscopy, confocal microscopy, ELISA, PCR, Western blot, generation of mouse chimerae.

Zaffaroni Mauro

Neurologo

Centro Studi Sclerosi Multipla

 Ospedale S. Antonio Abate, Gallarate

Via Pastori, 4
21013 Gallarate – Italia
Tel. 0331 751 386
Fax. 0331 751 385

Collaboratori
  • Damiano Baroncini, Neurologo
  • Francesca Grassivaro, Neurologo
  • Anna Bianchi, Biologa
  • Catia Stafanin, Biologa

Keywords su ambiti di competenza:
Sclerosi Multipla, Immunomodulazione.

 

Tecniche utilizzate:

IEF, Citoflurimetria, ELISA, PCR